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  5. 靜磁場抑制脂多醣引發小鼠產生生理與組織異常之研究
 
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靜磁場抑制脂多醣引發小鼠產生生理與組織異常之研究

Other Title
調查靜磁場減少生理和病理功能障礙的脂多醣攻擊小鼠
Type
thesis
Date Issued
2010-06-24
Author(s)
賴緯溢
Advisor
黃豪銘
Subjects
系所名稱:生醫材料暨工程研究所
Description
學位別:碩士
語文別:中文
指導教授:黃豪銘
共同指導教授:
口試委員:林哲堂;蕭裕源
中文關鍵字:靜磁場;脂質多醣體;過度免疫反應;BALB/cByJNarl小鼠
Abstract
彌散性血管內凝血( DIC)的是ㄧ種複雜的全身性凝血失調,涉及到血管內凝血和出血的症狀,過去的研究顯示,靜磁場(融資人)能有效的減緩脂多醣(LPS)所誘發的彌散性血管內凝血,但對其作用機制,目前仍未知,因此本研究的目的為探討靜磁場減緩脂多醣刺激小鼠死亡率的機制。
    在本研究中,以免疫BALB / cByNarl小鼠為試驗對象,以高劑量的脂多醣刺激並暴露於靜磁場(0.4 T)的環境作用下,再以小鼠的存活率和體溫變化進行觀察及紀錄。此外,觀察肝臟,肺臟和腎臟的活體組織切片來了解靜磁場對多重器官損傷的影響。我們的研究結果發現,靜磁場對脂多醣刺激小鼠之存活率有顯著性提升性(P < 0.05),且為控制組之存活率的1.5倍。另外在脂多醣注射六小時後,暴露於靜磁場的實驗組之小鼠平均體溫較控制組的平均體溫低1.07 ℃。此外,小鼠的活體組織切片之結果顯示,靜磁場可以降低小鼠的肝臟和腎臟因脂多醣造成的傷害。根據我們的結果,靜磁場對小鼠在受到脂多醣刺激後,有降低小鼠死亡率之影響,並且對彌散性血管內凝血的小鼠,有提升體溫控制能力的效果。
URI
https://203.71.86.71/handle/123456789/13127

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