雙相磷酸鈣骨基質之細胞反應研究

Purpose: This study developed calcium phosphate bone substitutes and their microstucture and in vitro cell response were evaluated in comparison with commercial hydroxyapatite (HA). Materials: HA powder was ballmilled and then sintered to transfer into the calcium phosphate bulks (CPB). The density, hardness, and microstructure of the CPB were investigated. The viability and proliferation of MG63 osteoblast-like cells on the commercial HA and the CPB were evaluated. Results: The x-ray diffraction confirmed that the CPB consisted of a-tricalcium phosphate (a-TCP), CaO, and HA. The hardness, density, and a-TCP-to-HA ratio of the CPB decreased when increasing the sintering duration. Cell tests demonstrated that the CPB exhibited an earlier cell-spread response than the commercial HA. Conclusions: This study demonstrated that a phase transformation of HA into a-TCP and CaO was achieved by sintering. The cell tests indicated that the CPB has favorable in vitro cellular performance, which implied that it presented potential as bone substitute.