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發展具抗凝結、抗菌與生物相容性之奈米薄膜於生醫之應用
Other Title
Development of Anticoagulation, Antibacterial and Biocompatible Nanostructured Films for Biomedical Applications
Type
thesis
Date Issued
2013-07-01
Author(s)
詹育豪
Advisor
黃瓊芳
Subjects
系所名稱：牙醫學系碩博士班
Description
學位別：博士
語文別：英文
指導教授：黃瓊芳
共同指導教授：歐耿良
口試委員：彭珮雯;張雍敏;劉沖明
中文關鍵字：奈米線;抗菌;抗凝;羥基磷灰石;磷酸三鈣;體外;燒結;C:H薄膜;銅摻雜;抗菌活性
語文別：英文
指導教授：黃瓊芳
共同指導教授：歐耿良
口試委員：彭珮雯;張雍敏;劉沖明
中文關鍵字：奈米線;抗菌;抗凝;羥基磷灰石;磷酸三鈣;體外;燒結;C:H薄膜;銅摻雜;抗菌活性
Abstract
The present research was divided into three parts; the first part of this study consisted of Ti implants to form anticoagulation and antibacterial films composed of carbon-based nanowires (NWs), the second part consisted of showing the biocompatibility of a bicalcium phosphate (BCP) bioceramic. The final part consisted of a discourse analysis of a nano-functionalized stainless steel.
Part I
Gas mixtures comprising H2:Ar:CH4 at various flow ratios were fed to a microwave plasma system during the preparation of Ti implants to form anticoagulation and antibacterial films composed of carbon-based nanowires (NWs). When the H2-flow ratio was increased, a phase transition (amorphization (α)->(α + quasi-α)->(α + quasi-α + NWs)->(α + quasi-α + NWs + multilayer-like NWs (MLNWs))->(NWs/MLNWs with nanograins)) was observed within the films formed on Ti during the microwave plasma discharge. In addition to producing a nanostructural film made of NWs, plasma carbonization caused the formation of an NW/nanograin phase that can enhance the hydrophobic and anticoagulation properties. Moreover, antibacterial tests also demonstrated the crucial role played by the hydrophobic films made of NWs in improving the antibacterial performance of the implants. The enhanced anticoagulation and antibacterial properties render the nanostructural films made of NWs as one of the promising materials that can be used in the heart valves, coronary artery stents, and dental implant surgical kits.
Part II
A bicalcium phosphate (BCP) bioceramic consisting of α-tricalcium phosphate (α-TCP) and hydroxyapatite (HA) was prepared by a two-step sintering. The microstructure of the BCP was investigated using scanning electron microscopy (SEM), transmission electron microscopy (TEM) and x-ray diffraction (XRD). The in vitro osteoinductivity was evaluated by testing the viability and proliferation of MG63 osteoblast-like cells on the BCP. Results show the BCP comprised α-TCP and HA and possessed the moderate hardness of 93.7 Hv. The cells spread faster on the BCP than those on the commercial HA. The earlier biological response of the BCP to MG63 cells suggested that the BCP is a potential bioceramic for enhancing osteoinductivity in vivo.
Part III
A hydrogenated Diamond-like carbon (a-C:H) with a copper dopant (Cu/a-C:H) was deposited on glass substrates using a combined radio-frequency plasma and magnetron sputtering deposition process under various Ar/CH4 gas mixtures. The effects of the Cu content on the structure and properties of the a-C:H matrix were investigated using X-ray diffraction (XRD), Raman transmission electron microscopy, high-resolution transmission electron microscopy (TEM), and nano-indentation. The bacterial activity of a Cu/a-C:H film was evaluated with Escherichia coli (E. coli). TEM images and XRD spectra demonstrated that composite films containing copper nanoparticles embedded in the a-C:H were deposited on the glass substrates. The Raman spectra showed the structure of a-C:H film was substantially changed by the incorporation of Cu. The Cu/a-C:H films offered superior antibacterial activity against E.coil indicating that they could be suitable for surface coatings in cardiovascular applications.
Part I
Gas mixtures comprising H2:Ar:CH4 at various flow ratios were fed to a microwave plasma system during the preparation of Ti implants to form anticoagulation and antibacterial films composed of carbon-based nanowires (NWs). When the H2-flow ratio was increased, a phase transition (amorphization (α)->(α + quasi-α)->(α + quasi-α + NWs)->(α + quasi-α + NWs + multilayer-like NWs (MLNWs))->(NWs/MLNWs with nanograins)) was observed within the films formed on Ti during the microwave plasma discharge. In addition to producing a nanostructural film made of NWs, plasma carbonization caused the formation of an NW/nanograin phase that can enhance the hydrophobic and anticoagulation properties. Moreover, antibacterial tests also demonstrated the crucial role played by the hydrophobic films made of NWs in improving the antibacterial performance of the implants. The enhanced anticoagulation and antibacterial properties render the nanostructural films made of NWs as one of the promising materials that can be used in the heart valves, coronary artery stents, and dental implant surgical kits.
Part II
A bicalcium phosphate (BCP) bioceramic consisting of α-tricalcium phosphate (α-TCP) and hydroxyapatite (HA) was prepared by a two-step sintering. The microstructure of the BCP was investigated using scanning electron microscopy (SEM), transmission electron microscopy (TEM) and x-ray diffraction (XRD). The in vitro osteoinductivity was evaluated by testing the viability and proliferation of MG63 osteoblast-like cells on the BCP. Results show the BCP comprised α-TCP and HA and possessed the moderate hardness of 93.7 Hv. The cells spread faster on the BCP than those on the commercial HA. The earlier biological response of the BCP to MG63 cells suggested that the BCP is a potential bioceramic for enhancing osteoinductivity in vivo.
Part III
A hydrogenated Diamond-like carbon (a-C:H) with a copper dopant (Cu/a-C:H) was deposited on glass substrates using a combined radio-frequency plasma and magnetron sputtering deposition process under various Ar/CH4 gas mixtures. The effects of the Cu content on the structure and properties of the a-C:H matrix were investigated using X-ray diffraction (XRD), Raman transmission electron microscopy, high-resolution transmission electron microscopy (TEM), and nano-indentation. The bacterial activity of a Cu/a-C:H film was evaluated with Escherichia coli (E. coli). TEM images and XRD spectra demonstrated that composite films containing copper nanoparticles embedded in the a-C:H were deposited on the glass substrates. The Raman spectra showed the structure of a-C:H film was substantially changed by the incorporation of Cu. The Cu/a-C:H films offered superior antibacterial activity against E.coil indicating that they could be suitable for surface coatings in cardiovascular applications.