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The Biological Effects of Photobiomodulation on Human Adipose Mesenchymal Stem Cells and Its Potential in Cell Therapy
Other Title
The Biological Effects of Photobiomodulation on Human Adipose Mesenchymal Stem Cells and Its Potential in Cell Therapy
Type
thesis
Date Issued
2022-06-23
Author(s)
NGUYEN LE THANH HANG
Advisor
潘力誠;張承仁
Subjects
系所名稱：生醫光機電研究所碩士班
Publisher
生醫光機電研究所碩士班
Description
口試委員：楊自森 Tzu-Sen Yang;呂隆昇 Long-Sheng Lu;張榮善 Jungshan Chang;張承仁 Cheng-Jen Chang ;潘力誠 Li-Chern Pan
網際網路,開放日期為2022-07-11
網際網路,開放日期為2022-07-11
Abstract
Background: Photobiomodulation (PBM) of human adipose-derived mesenchymal stem cells (hADSCs) using near-infrared (NIR) laser irradiation has recently emerged in cellular therapy as a potent alternative in promoting cell proliferation, migration, and differentiation during tissue regeneration.
Research purposes: A proposed single-cell near-infrared (NIR) laser irradiation system (830 nm) and the image-based approaches were established for investigating the modulatory effects in mitochondrial membrane potential (ΔΨm), reactive oxygen species (ROS), and vesicle transport in single living human adipose mesenchymal stem cells (hADSCs). In addition, the hADSC migration assay was examined at an optimal fluency to interpret the correlation between the PBM-induced increase in ΔΨm, ROS, vesicle transport, and cell migration.
Materials and Methods: The hADSCs were first seeded into a flow chamber and incubated for 24 hours. Rhodamine 123 (Rh123) and 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) were used to perform the ΔΨm and ROS stimulation on irradiated-hADSCs , respectively, with irradiation (830 nm) in the range of 2.5-10 (J/cm2), where time series of bright-field images were also obtained to identify the vesicle transport phenomenon. In addition, the PBM effect in stimulating the irradiated-hADSC movement at an optimal fluency (1.6 J/cm2) was investigated using culture-insert two wells in a 35 mm dish.
Results: Present results revealed that a fluence of 5 J/cm2 significantly promoted the ΔΨm, ROS, and vesicle transport phenomena compared to the control group (0 J/cm2) ) at 30 mins-post irradiation . Furthermore, the improvement in hADSC migration was statistically indicated between the PBM group (5 J/cm2) and the control group (0 J/cm2 + 10% FBS) at 24 hours post-irradiation.
Conclusion: Consequently, our single-cell research had demonstrated an increase of the ΔΨm, ROS, and vesicle transport at a fluence of 5 J/cm2 in the irradiated-hADSCs. Moreover, an improvement in hADSC movement was observed at an optimal fluency of 1.6 J/cm2. These data indicated the usage of PBM can to stimulate positive biological effects which results in stimulating cell proliferation, migration, and differentiation in stem cell-based therapy.
Research purposes: A proposed single-cell near-infrared (NIR) laser irradiation system (830 nm) and the image-based approaches were established for investigating the modulatory effects in mitochondrial membrane potential (ΔΨm), reactive oxygen species (ROS), and vesicle transport in single living human adipose mesenchymal stem cells (hADSCs). In addition, the hADSC migration assay was examined at an optimal fluency to interpret the correlation between the PBM-induced increase in ΔΨm, ROS, vesicle transport, and cell migration.
Materials and Methods: The hADSCs were first seeded into a flow chamber and incubated for 24 hours. Rhodamine 123 (Rh123) and 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) were used to perform the ΔΨm and ROS stimulation on irradiated-hADSCs , respectively, with irradiation (830 nm) in the range of 2.5-10 (J/cm2), where time series of bright-field images were also obtained to identify the vesicle transport phenomenon. In addition, the PBM effect in stimulating the irradiated-hADSC movement at an optimal fluency (1.6 J/cm2) was investigated using culture-insert two wells in a 35 mm dish.
Results: Present results revealed that a fluence of 5 J/cm2 significantly promoted the ΔΨm, ROS, and vesicle transport phenomena compared to the control group (0 J/cm2) ) at 30 mins-post irradiation . Furthermore, the improvement in hADSC migration was statistically indicated between the PBM group (5 J/cm2) and the control group (0 J/cm2 + 10% FBS) at 24 hours post-irradiation.
Conclusion: Consequently, our single-cell research had demonstrated an increase of the ΔΨm, ROS, and vesicle transport at a fluence of 5 J/cm2 in the irradiated-hADSCs. Moreover, an improvement in hADSC movement was observed at an optimal fluency of 1.6 J/cm2. These data indicated the usage of PBM can to stimulate positive biological effects which results in stimulating cell proliferation, migration, and differentiation in stem cell-based therapy.