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Effects of low-intensity pulsed ultrasound, dexamethasone/TGF-B1, and/or BMP-2 on the transcriptional Expression of genes in human mesenchymal stem cells: chondrogenic vs. osteogenic differentiation.
Type
article
Date Issued
2010-06
Author(s)
Chien-Hung Lai, Shih-Ching Chen+, Li-Hsuan Chiu, Charng-Bin Yang, Yu-Hui Tsai, Chun S. Zuo, Walter Hong-Shong Chang, Wen-Fu Lai.
Subjects
學科：復健學科
期刊論文
Abstract
The effectsoflow-intensitypulsedultrasound(LIPUS)onthedifferentiationofhumanmesenchymal
stem cells(hMSCs)wereinvestigatedinthisstudy.hMSCsweresubjectedtoLIPUSwithorwithoutdexametha-
sone/transforminggrowthfactor-b1 (TD)orbonemorphogeneticprotein-2(BMP-2)andtheeffectsofthistreat-
ment wereassessed.TD-treatedhMSCsexhibitedcharacteristicchondrogenicmorphologyandincreased
messenger RNA(mRNA)expressionofchondrogenicmarkersandLIPUSenhancedthechondrogenicdifferenti-
ation ofhMSCstreatedwithTD.TheexpressionofRunx2,anosteogenictranscriptionfactorwasnotalteredin
either TDtreatmentgroup;however,asignificantincreasewasdetectedintheLIPUSonlygroup.Theosteogenic
appearance exhibited3daysafterLIPUSand/orBMP-2treatment.IncreasesinthemRNAexpressionlevelsof
osteogenic markers,Runx2andALPwerealsodetected.Therewasnoadditiveoralteredeffectwithcombined
LIPUS andBMP-2treatment.LIPUSalonecanincreaseosteogenicdifferentiationofhMSCsandLIPUSenhances
TD-mediated chondrogenicdifferentiationofhMSCs.Clinically,LIPUSmaydifferentiallyinfluencebonevs.
cartilage repair.(E-mail: whchang@cycu.edu.tw or laitw@tmu.edu.tw or tlai@mclean.harvard.edu) 2010
WorldFederationforUltrasoundinMedicine&Biology.
stem cells(hMSCs)wereinvestigatedinthisstudy.hMSCsweresubjectedtoLIPUSwithorwithoutdexametha-
sone/transforminggrowthfactor-b1 (TD)orbonemorphogeneticprotein-2(BMP-2)andtheeffectsofthistreat-
ment wereassessed.TD-treatedhMSCsexhibitedcharacteristicchondrogenicmorphologyandincreased
messenger RNA(mRNA)expressionofchondrogenicmarkersandLIPUSenhancedthechondrogenicdifferenti-
ation ofhMSCstreatedwithTD.TheexpressionofRunx2,anosteogenictranscriptionfactorwasnotalteredin
either TDtreatmentgroup;however,asignificantincreasewasdetectedintheLIPUSonlygroup.Theosteogenic
appearance exhibited3daysafterLIPUSand/orBMP-2treatment.IncreasesinthemRNAexpressionlevelsof
osteogenic markers,Runx2andALPwerealsodetected.Therewasnoadditiveoralteredeffectwithcombined
LIPUS andBMP-2treatment.LIPUSalonecanincreaseosteogenicdifferentiationofhMSCsandLIPUSenhances
TD-mediated chondrogenicdifferentiationofhMSCs.Clinically,LIPUSmaydifferentiallyinfluencebonevs.
cartilage repair.(E-mail: whchang@cycu.edu.tw or laitw@tmu.edu.tw or tlai@mclean.harvard.edu) 2010
WorldFederationforUltrasoundinMedicine&Biology.
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