樟芝(ST1)應用於骨損傷之骨再生研究

Antrodia camphorata (A C) is a medicinal fungus that has previously demonstrated many beneficial properties such as cytotoxicity to cancer cells and amelioration of inflammation syndromes. This study evaluates pure compound extracts of A C for osteogenesis, osteoporosis recovery, and osteoporotic bone injury healing effects in vitro with preosteoblast cells (MC3T3) and in vivo with an osteoporosis mouse model established in our previous studies, ovariectomied senescence accelerated mice (OVX-SAMP8). This study demonstrated that ST1 is a well-defined pure compound of A C that exhibits low cytotoxicity to preosteoblasts at 25 ug/mL, while osteogenic gene expression (RUNX2, OPN and OCN) is significantly up-regulated, and revealed an increased ratio of OPG to RANKL, indicating maintenance of the bone matrix through inhibition of osteoclastic pathway. Additionally, evaluation by Alizarin Red S staining showed increased mineralization in ST1 treated preosteoblasts. In vitro results show that ST1 could promote osteogenesis and inhibit bone digestion. In vivo testing examined both long term and short term effects of ST1 on OVX-SAMP8 mice. Short term treatment was evaluated on an osteoporotic bone injury model. Our results showed that ST1 administered by i.p. injection every other day for 2 weeks following a bone injury improved the rate of bone healing when compared to Sham group. In our long term study, OVX-SAMP8 mice were treated orally with ST1 every other day for 4 months. Osteogenesis was improved in BMSCs from the ST1 treated mice, however, possible side effects were observed. ST1 could be considered for short term osteoporosis or osteoporotic fracture therapy through anabolic action on bone health.