Yu-Chia HsiehWen-Sen LeeTsong-Yih OuPo-Ren Hsueh2026-05-272026-05-272009https://203.71.86.71/handle/123456789/38561From May 2007 to January 2008, 30 isolates of vancomycin-resistant enterococci (VRE), including 29 Enterococcus faecium (96.7%) and 1 E. faecalis (3.3%) were obtained from various clinical specimens of 30 patients treated at a university hospital in Taiwan. Among these patients, 27 had VRE infections, including urinary tract infection (n=16), bacteremia (n=5), wound infection (n=5), and central nervous system infection (n=1). Three patients had VRE colonization. All of these isolates belonged to the vanA genotype with vancomycin minimum inhibitory concentrations of 64≥128 μg/ml. The isolate of E. faecalis had VanB phenotype-vanA genotype. All these isolates were susceptible to linezolid and were inhibited by tigecycline at 0.25 μg/ml. Multilocus sequence typing (MLST) analysis of the E. faecium isolates showed that 82.8% were ST78, which belongs to lineage C1. Transposon typing classified the 30 isolates of VRE into three types and most of the Tn1546-like elements contained an IS1251- like insertion sequence. Mating experiments showed that the vanA gene clusters were transferable at a frequency of about 10−6 to 10−7. Our findings indicate that nosocomial spread of VRE resulted from dissemination of lineage C1 E. faecium clones, including a novel E. faecium MLST type(ST444), and the horizontal transfer of Tn1546 elements among enterococci.91733 bytesapplication/pdfen-US萬芳醫院科部：內科部類別：期刊論文article