Chien-Hung Lai, Shih-Ching Chen+, Li-Hsuan Chiu, Charng-Bin Yang, Yu-Hui Tsai, Chun S. Zuo, Walter Hong-Shong Chang, Wen-Fu Lai.Shih-Ching Chen+, Li-Hsuan Chiu, Charng-Bin Yang, Yu-Hui Tsai, Chun S. Zuo, Walter Hong-Shong Chang, Wen-Fu Lai.Chien-Hung Lai2026-05-202026-05-202010-06https://203.71.86.71/handle/123456789/19912The effectsoflow-intensitypulsedultrasound(LIPUS)onthedifferentiationofhumanmesenchymal stem cells(hMSCs)wereinvestigatedinthisstudy.hMSCsweresubjectedtoLIPUSwithorwithoutdexametha- sone/transforminggrowthfactor-b1 (TD)orbonemorphogeneticprotein-2(BMP-2)andtheeffectsofthistreat- ment wereassessed.TD-treatedhMSCsexhibitedcharacteristicchondrogenicmorphologyandincreased messenger RNA(mRNA)expressionofchondrogenicmarkersandLIPUSenhancedthechondrogenicdifferenti- ation ofhMSCstreatedwithTD.TheexpressionofRunx2,anosteogenictranscriptionfactorwasnotalteredin either TDtreatmentgroup;however,asignificantincreasewasdetectedintheLIPUSonlygroup.Theosteogenic appearance exhibited3daysafterLIPUSand/orBMP-2treatment.IncreasesinthemRNAexpressionlevelsof osteogenic markers,Runx2andALPwerealsodetected.Therewasnoadditiveoralteredeffectwithcombined LIPUS andBMP-2treatment.LIPUSalonecanincreaseosteogenicdifferentiationofhMSCsandLIPUSenhances TD-mediated chondrogenicdifferentiationofhMSCs.Clinically,LIPUSmaydifferentiallyinfluencebonevs. cartilage repair.(E-mail: whchang@cycu.edu.tw or laitw@tmu.edu.tw or tlai@mclean.harvard.edu) 2010 WorldFederationforUltrasoundinMedicine&Biology.1501878 bytesapplication/pdfen-US學科：復健學科期刊論文article