歐耿良后淑娟2026-06-112026-06-112013-12-22https://203.71.86.71/handle/123456789/56921學位別：碩士 語文別：英文 指導教授：歐耿良 共同指導教授： 口試委員：林利香;劉沖明 中文關鍵字：DLC膜To evaluate the cell responses of titanium oxide layer with the nanoporous micro-structure, an electrochemical anodization approach was proposed to create a nanoporous structure that superimposed onto the moderately rough sand-blasted, large-grit, acid-etched (SLA) Ti surface (denoted as SLAffinity-Ti). The osteoblast-like cells were cultured on SLAffinity-Ti and un-treated surfaces. Cell adhesion, viability,mRNA expressions as well as the productions of alkaline phosphatase (ALP) and osteocalcin (OC) were evaluated at predetermined intervals. The present results showed that cells had a high viability, attachment, and improved cell proliferation at day 7 and 14 when cultured on SLAffinity-Ti surface. The mRNA expressions of ALP and OC were upregulated while the type I collagen was not influenced. Additionally, the cells cultured on the SLAffinity-Ti surface produced a higher level of ALP and OC relative to cells on untreated surfaces. These findings confirmed that surface topography (nanoporous roughness) and TiO2 layers influenced the cell responses and the study revealed that cells grown on SLAffinity-Ti may improve osseointegration for Ti implants.系所名稱：醫療器材產業碩士專班thesis