黃瓊芳后秉仁2026-06-112026-06-112013-12-22https://203.71.86.71/handle/123456789/56920學位別：碩士 語文別：英文 指導教授：黃瓊芳 共同指導教授： 口試委員：張雍敏;劉沖明 中文關鍵字：MG-63細胞，鈦6Al-4VIn the present study, the influence of the discharge current in the powder mixed electrical discharge machining (PMEDM) process on the deposition of an electrical-discharge layer onto titanium-based alloys (Ti–6Al–4V (Ti64)) as well as the influence of such a deposited biocompatible electrical-discharge layer on MG-63 osteoblast-like cells were investigated. An electrical-discharge layer was deposited on Ti64 specimens using a die-sinking electric discharge machine with titanium dioxide (TiO2) powder suspended in distilled water. MG-63 cells were cultured on untreated and PMEDM-treated specimens. Cell morphology and metabolic activity were determined. PMEDM treatment roughened the surface on a micro scale, whereas the nano-scale pores were superimposed, accompanying with the increased TiO2 content. Cells seeded on the PMEDM-15 surfaces showed that the cells were flattened and well spread out and a significant increase in their proliferation on PMEDM-treated surfaces as compared with those seeded on the untreated surfaces. Our study revealed that the biological properties and microstructure of the PMEDM-treated surfaces, including their chemical composition and surface roughness, play important roles in their II osteoblastic responses to the cultured specimens. These results can provide insights into the development of new biomedical implant surfaces.系所名稱：醫療器材產業碩士專班thesis